By Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)
The annual assembly of the ecu Tissue tradition ., Society was once held on the fort of Zinkovy in Czechoslovakia from may possibly 7-10,1969. integrated as a part of this assembly used to be a symposium on "Aging in mobile and Tissue Culture." This quantity includes the papers offered at that symposium. using mobile and tissue tradition ideas to review the mechanism of getting older isn't new. for instance, it has lengthy been recognized that age-associated alterations which take place in plasma can inhibit phone proliferation in vitro; additionally that the time lapse sooner than phone migration from ex planted tissue fragments raises with expanding age. those are either examples of the expression in vitro of getting older in vivo. extra lately, realization has been concerned about the prevalence of senescence in vitro. those investi gations have incorporated experiences of adjustments in non dividing telephone cultures, and to a a bit higher volume, of age-related alterations within the proliferative ability of cells in vitro. for instance, cells derived from human fetal lung continue many homes of standard cells together with a reliable common diploid karyotype and those cultures were proven to have a restricted life-span in vitro. In addi tion, cultures derived from human grownup lung exhibit an analogous common features and seem to have a shorter lifestyles span than cells derived from fetal lung.
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Additional info for Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969
In this experiment it is possible to correlate these fluctuations with culture conditions because there is a strict up-and-down pattern. Until the 26th passage these cell strains were subcultured twice a week and the growth periods were three and four days respectively. The length of these growth periods clearly have an influence on the size of the cells in the next passage two days after seeding. The subcultured cells show a larger volume two days after seeding when the preceding growth period was three days than when the preceding growth period lasted four days.
L. Hayflick,"The limited in vitro lifetime of human diploid cell strains," Exp. , 37: 614, 1965. L. Hayflick, "Cell culture and the aging phenomenon, " in: Topics in the Biology of Aging, New York, Interscience, 1966, p. 83. V. J. Cristofalo, "Metabolic aspects of ageing in euploid human cells," This volume and personal communication. L. E. Orgel, "The maintenance of the accuracy of protein synthesis and its relevance to ageing," Proc. Natl. Acad. , 49: 517, 1963. P. D. Cooper, "The plaque assay of animal viruses, " in: Methods in Virology, Vol.
Detailed studies of the growth characteristics and invasive potential of similar euploid strains at various times after isolation could be extremely enlightening at this point. The above discussion of results from studies with cell strains from various species gives rise to several related questions. J. HAY hypotheses suggested to explain the phenomenon of limited cell proliferation in vitro. Current culture conditions have been developed largely as a result of the nutritional requirements of permanent cell lines.
Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969 by Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)